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Clinical significance of markers of collagen metabolism in rheumatic mitral valve disease.
[acute rheumatic fever]
Rheumatic
Heart
Disease
(
RHD
)
,
a
chronic
acquired
heart
disorder
results
from
Acute
Rheumatic
Fever
.
It
is
a
major
public
health
concern
in
developing
countries
.
In
RHD
,
mostly
the
valves
get
affected
.
The
present
study
investigated
whether
extracellular
matrix
remodelling
in
rheumatic
valve
leads
to
altered
levels
of
collagen
metabolism
markers
and
if
such
markers
can
be
clinically
used
to
diagnose
or
monitor
disease
progression
.
This
is
a
case
control
study
comprising
118
subjects
.
It
included
77
cases
and
41
healthy
controls
.
Cases
were
classified
into
two
groups-
Mitral
Stenosis
(
MS
)
and
Mitral
Regurgitation
(
MR
)
.
Carboxy-terminal
propeptide
of
type
I
procollagen
(
PICP
)
,
amino-terminal
propeptide
of
type
III
procollagen
(
PIIINP
)
,
total
Matrix
Metalloproteinase-
1
(
MMP-
1
)
and
Tissue
Inhibitor
of
Metalloproteinase-
1
(
TIMP-
1
)
were
assessed
.
Histopathology
studies
were
performed
on
excised
mitral
valve
leaflets
.
A
p
value
<
0
.
05
was
considered
statistically
significant
.
Plasma
PICP
and
PIIINP
concentrations
increased
significantly
(
p
<
0
.
01
)
in
MS
and
MR
subjects
compared
to
controls
but
decreased
gradually
over
a
one
year
period
post
mitral
valve
replacement
(
p
<
0
.
05
)
.
In
MS
,
PICP
level
and
MMP-
1
/
TIMP-
1
ratio
strongly
correlated
with
mitral
valve
area
(
r
 
=
 
-
0
.
40
;
r
 
=
 
0
.
49
respectively
)
and
pulmonary
artery
systolic
pressure
(
r
 
=
 
0
.
49
;
r
 
=
 
-
0
.
49
respectively
)
;
while
in
MR
they
correlated
with
left
ventricular
internal
diastolic
(
r
 
=
 
0
.
68
;
r
 
=
 
-
0
.
48
respectively
)
and
systolic
diameters
(
r
 
=
 
0
.
65
;
r
 
=
 
-
0
.
55
respectively
)
.
Receiver
operating
characteristic
curve
analysis
established
PICP
as
a
better
marker
(
AUC
 
=
 
0
.
95
;
95
%
CI
 
=
 
0
.
91
-
0
.
99
;
p
<
0
.
0001
)
.
A
cut-off
>
459
ng
/
mL
for
PICP
provided
91
%
sensitivity
,
90
%
specificity
and
a
likelihood
ratio
of
9
in
diagnosing
RHD
.
Histopathology
analysis
revealed
inflammation
,
scarring
,
neovascularisation
and
extensive
leaflet
fibrosis
in
diseased
mitral
valve
.
Levels
of
collagen
metabolism
markers
correlated
with
echocardiographic
parameters
for
RHD
diagnosis
.
Diseases
Validation
Diseases presenting
"altered levels of collagen metabolism markers"
symptom
acute rheumatic fever
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