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A random Abstract
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Molecular Genotyping of Toxoplasma gondii in Human Spontaneous Aborted Fetuses in Shiraz, Southern Iran.
[congenital toxoplasmosis]
Congenital
toxoplasmosis
is
associated
with
variable
complications
including
encephalitis
,
microcephaly
,
hydrocephaly
,
hepatitis
,
lymphadenopathy
and
even
intrauterine
death
.
Presence
of
Toxoplasma
gondii
in
human
placenta
may
induce
congenital
infection
.
The
aim
of
this
study
was
to
determine
the
genotypes
of
Toxoplasma
gondii
infection
in
human
spontaneous
aborted
fetuses
in
Shiraz
,
south
of
Iran
.
F
ive
hundred
and
forty
two
paraffin-embedded
blocks
of
aborted
placenta
were
collected
,
from
two
university-affiliated
hospitals
in
Shiraz
.
Occurrence
of
spontaneous
abortion
was
confirmed
by
examine
of
the
slides
.
After
re
-cutting
of
the
blocks
and
dewaxing
,
semi-nested
PCR
assay
was
used
to
detect
the
fragments
of
T
.
gondii
B
1
gene
in
the
samples
.
Also
direct
molecular
genotyping
was
performed
on
positive
samples
with
Restriction
Fragment
Length
Polymorphism-
PCR
analysis
on
the
SAG
2
gene
.
Among
the
542
tissue
samples
,
the
B
1
gene
was
amplified
from
78
(
14
.
4
%
)
of
cases
with
the
semi
nested
PCR
and
typed
by
RFLP
.
The
genotype
of
Toxoplasma
strains
of
65
(
out
of
78
)
PCR-
positive
samples
were
evaluated
and
54
out
of
65
(
83
.
1
%
)
were
found
to
be
type
II
and
11
out
of
65
(
16
.
9
%
)
were
type
I
.
Considering
the
high
level
of
Toxoplasma
infection
in
aborted
fetuses
in
this
study
,
Toxoplasma
might
largely
contribute
to
spontaneous
abortion
in
this
area
of
Iran
.
