Rare Diseases Symptoms Automatic Extraction
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Genotyping of samples from German patients with ocular, cerebral and systemic toxoplasmosis reveals a predominance of Toxoplasma gondii type II.
[congenital toxoplasmosis]
Toxoplasmosis
is
an
important
zoonosis
transmitted
from
animals
to
humans
world-
wide
.
In
order
to
determine
Toxoplasma
gondii
genotypes
in
individuals
living
in
Germany
and
to
compare
findings
with
those
in
animals
,
we
analysed
nine
independent
and
unlinked
genetic
markers
(
nSAG
2
,
SAG
3
,
BTUB
,
GRA
6
,
c
22
-
8
,
c
29
-
2
,
L
358
,
PK
1
and
Apico
)
by
PCR-RFLP
in
83
archived
T
.
gondii-
positive
DNA
samples
from
patients
with
ocular
toxoplasmosis
(
n
=
35
)
,
toxoplasmic
encephalitis
(
n
=
32
)
,
systemic
toxoplasmosis
after
bone
-marrow
transplantation
(
n
=
15
)
and
congenital
toxoplasmosis
(
n
=
1
)
.
In
46
of
these
83
samples
the
presence
of
T
.
gondii
DNA
was
confirmed
by
conventional
end-point
PCR
.
Among
these
,
17
T
.
gondii-
positive
samples
were
typed
at
all
nine
loci
.
The
majority
(
15
/
17
,
88
.
2
%
)
of
these
samples
were
of
T
.
gondii
type
II
(
i
.
e
.
,
including
both
,
the
Apico
type
II
and
Apico
type
I
variants
)
.
In
addition
,
in
one
sample
a
T
.
gondii
type
II
/
type
III
allele
combination
and
in
another
sample
a
T
.
gondii
genotype
displaying
type
III
alleles
at
all
markers
was
observed
.
In
the
remaining
11
samples
,
in
which
T
.
gondii
could
only
be
partially
typed
,
exclusively
type
II
(
n
=
10
)
or
type
III
(
n
=
1
)
alleles
were
observed
.
Results
of
the
present
study
suggest
that
the
majority
of
patients
in
Germany
are
infected
with
type
II
T
.
gondii
regardless
of
the
clinical
manifestation
of
toxoplasmosis
.
This
finding
is
in
accord
with
the
predominance
of
type
II
T
.
gondii
in
oocysts
isolated
from
cats
and
in
tissues
of
other
intermediate
hosts
in
Germany
.
Diseases
Validation
Diseases presenting
"encephalitis"
symptom
alexander disease
congenital toxoplasmosis
dracunculiasis
locked-in syndrome
malignant atrophic papulosis
pyomyositis
pyruvate dehydrogenase deficiency
scrub typhus
systemic capillary leak syndrome
typhoid
This symptom has already been validated