Rare Diseases Symptoms Automatic Extraction
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Freezing and storage at -20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis.
[congenital toxoplasmosis]
Nucleic
acid-based
testing
has
become
crucial
for
toxoplasmosis
diagnosis
.
For
retrospective
(
forensic
or
scientific
)
studies
,
optimal
methods
must
be
employed
for
DNA
long
-term
storage
.
We
compared
Toxoplasma
gondii
detection
before
and
after
DNA
storage
using
real-time
PCR
.
No
significant
differences
were
found
depending
on
duration
or
storage
conditions
at
-
20
°
C
or
-
80
°
C
.