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Diagnostic value of a Rec-ELISA using Toxoplasma gondii recombinant SporoSAG, BAG1, and GRA1 proteins in murine models infected orally with tissue cysts and oocysts.
[congenital toxoplasmosis]
Toxoplasma
gondii
causes
congenital
toxoplasmosis
in
newborns
resulting
with
fetal
anomalies
.
Determining
the
initiation
time
of
infection
is
very
important
for
pregnant
women
and
current
serological
assays
have
drawbacks
in
distinguishing
the
recently
acute
toxoplasmosis
.
Diagnosis
of
recently
acute
infection
may
be
improved
by
using
stage
specific
antigens
in
serological
assays
.
In
the
present
study
,
the
diagnostic
value
of
sporozoite
specific
SporoSAG
,
bradyzoite
specific
BAG
1
proteins
and
GRA
1
protein
expressed
by
all
forms
of
the
parasite
have
been
evaluated
ELISA
using
sera
systematically
collected
from
mice
administered
orally
with
tissue
cyst
and
oocysts
.
The
anti-
SporoSAG
IgM
antibodies
in
sera
obtained
from
mice
infected
with
oocysts
peaked
significantly
at
days
1
,
10
,
and
15
(
P
<
0
.
01
)
.
The
anti-
BAG
1
IgM
antibodies
in
sera
obtained
from
mice
infected
with
tissue
cysts
peaked
significantly
at
days
15
,
40
,
and
120
(
P
<
0
.
05
)
.
The
anti-
GRA
1
IgM
antibodies
in
sera
obtained
from
mice
infected
with
oocysts
peaked
significantly
at
days
2
,
10
,
and
40
(
P
<
0
.
01
)
.
The
anti-
GRA
1
IgM
antibodies
in
sera
obtained
from
mice
infected
with
tissue
cysts
peaked
significantly
only
at
day
40
(
P
<
0
.
05
)
.
The
anti-
SporoSAG
,
anti-
BAG
1
,
and
anti-
GRA
1
IgG
titers
of
mice
showed
significant
increases
at
day
40
(
P
<
0
.
05
)
and
decrement
started
for
only
anti-
GRA
1
IgG
at
day
120
.
The
presence
of
anti-
SporoSAG
IgM
and
IgG
antibodies
can
be
interpreted
as
recently
acute
infection
between
days
10
-
40
because
IgM
decreases
at
day
40
.
Similarly
,
presence
of
anti-
BAG
1
IgM
and
absence
of
IgG
can
be
evaluated
as
a
recently
acute
infection
that
occurred
40
days
before
because
IgG
peaks
at
day
40
.
A
peak
in
anti-
GRA
1
antibody
level
at
first
testing
and
reduction
in
consecutive
sample
can
be
considered
as
an
infection
approximately
around
day
40
or
prior
.
Overall
,
recombinant
SporoSAG
,
BAG
1
and
GRA
1
proteins
can
be
accepted
as
valuable
diagnostic
markers
of
recently
acute
toxoplasmosis
.
Diseases
Validation
Diseases presenting
"reduction in consecutive sample can be considered as an infection approximately around day 40 or prior"
symptom
congenital toxoplasmosis
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