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Multicentric comparative assessment of the Bio-Evolution® Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for the molecular diagnosis of congenital toxoplasmosis.
[congenital toxoplasmosis]
Detection
of
Toxoplasma
gondii
in
amniotic
fluid
is
an
essential
tool
for
the
prenatal
diagnosis
of
congenital
toxoplasmosis
and
today
is
essentially
based
upon
PCR
.
Although
some
consensus
is
emerging
,
this
molecular
diagnosis
suffers
from
a
lack
of
standardization
and
an
extreme
diversity
of
laboratory-developed
methods
.
Commercial
kits
for
the
detection
of
T
.
gondii
by
PCR
have
been
recently
developed
and
offer
certain
advantages
;
however
they
must
be
assessed
in
comparison
with
optimized
reference
PCR
assays
.
The
present
multicentric
study
aimed
at
comparing
the
Bio-
Evolution
®
Toxoplasma
gondii
detection
kit
with
laboratory-developed
PCR
assays
set
up
in
eight
proficient
centers
in
France
.
The
study
compared
157
amniotic
fluid
samples
and
found
a
concordance
of
99
%
and
100
%
using
76
T
.
gondii-infected
samples
and
81
uninfected
samples
,
respectively
.
Moreover
,
taking
into
account
the
classification
of
the
European
Research
Network
on
Congenital
Toxoplasmosis
,
the
overall
diagnostic
sensitivity
of
all
assays
was
identical
and
calculated
at
86
%
(
54
/
63
)
;
specificity
was
100
%
for
all
.
Finally
,
the
relative
quantification
results
were
in
good
agreement
between
the
kit
and
the
laboratory-developed
assays
.
The
good
performances
of
this
commercial
kit
are
probably
in
part
linked
to
the
use
of
a
number
of
good
practices
:
detection
in
multiplicate
,
amplification
of
the
repetitive
DNA
target
'
re
p
529
'
,
use
of
an
internal
control
for
the
detection
of
PCR
inhibitors
.
The
only
drawbacks
noted
at
the
time
of
the
study
were
the
absence
of
Uracil-
N-
glycosylase
,
as
well
as
small
defects
in
the
reliability
of
the
production
of
different
reagents
.
Diseases
Validation
Diseases presenting
"prenatal diagnosis"
symptom
22q11.2 deletion syndrome
achondroplasia
adrenomyeloneuropathy
alexander disease
alpha-thalassemia
aromatase deficiency
benign recurrent intrahepatic cholestasis
cadasil
canavan disease
classical phenylketonuria
cohen syndrome
congenital adrenal hyperplasia
congenital diaphragmatic hernia
congenital toxoplasmosis
cystinuria
dentinogenesis imperfecta
epidermolysis bullosa simplex
harlequin ichthyosis
holt-oram syndrome
homocystinuria without methylmalonic aciduria
hydrocephalus with stenosis of the aqueduct of sylvius
junctional epidermolysis bullosa
kindler syndrome
krabbe disease
lamellar ichthyosis
megacystis-microcolon-intestinal hypoperistalsis syndrome
monosomy 21
neonatal adrenoleukodystrophy
oculocutaneous albinism
omenn syndrome
phenylketonuria
primary hyperoxaluria type 1
pyruvate dehydrogenase deficiency
severe combined immunodeficiency
wolf-hirschhorn syndrome
x-linked adrenoleukodystrophy
zellweger syndrome
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