A novel method for the large-scale production of PG-CNP37, a C-type natriuretic peptide analogue.

[achondroplasia]

Achondroplasia is the most common form of human dwarfism caused by a mutation in the fibroblast growth factor receptor 3 (FGFR 3 ), resulting in abnormal endochondral bone formation . C-type natriuretic peptide (CNP ) is a potent stimulator of endochondral bone growth and represents a potential therapy for achondroplasia . We have developed a novel , simple and cost effective method to produce a CNP analogue , PG-CNP 37 , at a large scale from Escherichia coli . A PG-CNP 37 fusion protein was over-expressed as inclusion bodies in E . coli , which were purified then cleaved by formic acid to release the PG-CNP 37 peptide . Approximately 0 .5 g of 95 % pure , soluble and active PG-CNP 37 peptide was produced from 1 L of culture using this method and may represent a viable means for large -scale production of other therapeutic peptides .

Diseases
Validation

Diseases presenting "common form" symptom

achondroplasia

alexander disease

benign recurrent intrahepatic cholestasis

cadasil

child syndrome

classical phenylketonuria

cutaneous mastocytosis

erythropoietic protoporphyria

familial mediterranean fever

hereditary cerebral hemorrhage with amyloidosis

hodgkin lymphoma, classical

holt-oram syndrome

homocystinuria without methylmalonic aciduria

oral submucous fibrosis

pendred syndrome

primary hyperoxaluria type 1

severe combined immunodeficiency

thoracic outlet syndrome

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