A novel method for the large-scale production of PG-CNP37, a C-type natriuretic peptide analogue.

[achondroplasia]

Achondroplasia is the most common form of human dwarfism caused by a mutation in the fibroblast growth factor receptor 3 (FGFR 3 ), resulting in abnormal endochondral bone formation . C-type natriuretic peptide (CNP ) is a potent stimulator of endochondral bone growth and represents a potential therapy for achondroplasia . We have developed a novel , simple and cost effective method to produce a CNP analogue , PG-CNP 37 , at a large scale from Escherichia coli . A PG-CNP 37 fusion protein was over-expressed as inclusion bodies in E . coli , which were purified then cleaved by formic acid to release the PG-CNP 37 peptide . Approximately 0 .5 g of 95 % pure , soluble and active PG-CNP 37 peptide was produced from 1 L of culture using this method and may represent a viable means for large -scale production of other therapeutic peptides .

Diseases
Validation

Diseases presenting "bone formation" symptom

achondroplasia

aromatase deficiency

dedifferentiated liposarcoma

dentin dysplasia

dentinogenesis imperfecta

pleomorphic liposarcoma

well-differentiated liposarcoma

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