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Autofluorescence of the cells in human subretinal fluid.
[coats disease]
The
origin
of
autofluorescence
in
the
subretinal
space
and
the
autofluorescence
properties
of
the
cells
were
investigated
in
surgically
collected
subretinal
fluid
.
Subretinal
fluid
was
surgically
collected
from
four
eyes
of
patients
with
rhegmatogenous
retinal
detachment
(
three
eyes
)
and
Coats
'
disease
(
one
eye
)
.
After
cytocentrifuge
preparation
of
the
cells
in
the
fluid
and
immunofluorescence
staining
,
a
cytologic
examination
was
conducted
by
using
confocal
scanning
laser
microscopy
.
The
autofluorescence
of
the
cells
was
elucidated
by
measuring
the
fluorescence
spectra
with
spectroscopy
,
to
obtain
different
excitation
laser
light
emission
fingerprints
.
The
cells
from
the
subretinal
fluid
were
classified
into
three
types
:
CD
68
-
negative
cells
containing
numerous
pigmented
granules
,
CD
68
-
positive
cells
containing
few
pigments
,
and
CD
68
-
negative
cells
with
no
pigmented
granules
.
Autofluorescence
was
observed
in
the
inclusions
of
the
cells
classified
into
the
former
two
types
.
When
the
cells
were
excited
by
a
458
-
or
488
-
nm
laser
light
,
emission
spectra
in
autofluorescence
showed
little
difference
between
CD
68
-
positive
and
-
negative
cells
.
Peak
analysis
confirmed
that
the
two
types
of
cells
showed
the
same
emission
peaks
within
this
range
of
excitation
light
.
Autofluorescent
inclusions
appeared
in
the
CD
68
-
positive
and
-
negative
cells
in
the
subretinal
fluid
.
The
macrophages
in
the
subretinal
fluid
possess
autofluorescence
that
is
spectroscopically
similar
to
lipofuscin
.
Autofluorescence
of
macrophages
can
be
attributed
to
degenerated
outer
segments
and
debris
from
apoptotic
photoreceptors
.
Clinicians
should
consider
migration
of
macrophages
,
in
addition
to
retinal
pigment
epithelium
,
as
the
possible
source
when
abnormal
fundus
autofluorescence
is
observed
using
an
ordinary
set
of
fluorescence
filters
.
Diseases
Validation
Diseases presenting
"retinal pigment epithelium"
symptom
coats disease
oculocutaneous albinism
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