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Autofluorescence of the cells in human subretinal fluid.
[coats disease]
The
origin
of
autofluorescence
in
the
subretinal
space
and
the
autofluorescence
properties
of
the
cells
were
investigated
in
surgically
collected
subretinal
fluid
.
Subretinal
fluid
was
surgically
collected
from
four
eyes
of
patients
with
rhegmatogenous
retinal
detachment
(
three
eyes
)
and
Coats
'
disease
(
one
eye
)
.
After
cytocentrifuge
preparation
of
the
cells
in
the
fluid
and
immunofluorescence
staining
,
a
cytologic
examination
was
conducted
by
using
confocal
scanning
laser
microscopy
.
The
autofluorescence
of
the
cells
was
elucidated
by
measuring
the
fluorescence
spectra
with
spectroscopy
,
to
obtain
different
excitation
laser
light
emission
fingerprints
.
The
cells
from
the
subretinal
fluid
were
classified
into
three
types
:
CD
68
-
negative
cells
containing
numerous
pigmented
granules
,
CD
68
-
positive
cells
containing
few
pigments
,
and
CD
68
-
negative
cells
with
no
pigmented
granules
.
Autofluorescence
was
observed
in
the
inclusions
of
the
cells
classified
into
the
former
two
types
.
When
the
cells
were
excited
by
a
458
-
or
488
-
nm
laser
light
,
emission
spectra
in
autofluorescence
showed
little
difference
between
CD
68
-
positive
and
-
negative
cells
.
Peak
analysis
confirmed
that
the
two
types
of
cells
showed
the
same
emission
peaks
within
this
range
of
excitation
light
.
Autofluorescent
inclusions
appeared
in
the
CD
68
-
positive
and
-
negative
cells
in
the
subretinal
fluid
.
The
macrophages
in
the
subretinal
fluid
possess
autofluorescence
that
is
spectroscopically
similar
to
lipofuscin
.
Autofluorescence
of
macrophages
can
be
attributed
to
degenerated
outer
segments
and
debris
from
apoptotic
photoreceptors
.
Clinicians
should
consider
migration
of
macrophages
,
in
addition
to
retinal
pigment
epithelium
,
as
the
possible
source
when
abnormal
fundus
autofluorescence
is
observed
using
an
ordinary
set
of
fluorescence
filters
.
Diseases
Validation
Diseases presenting
"positive cells"
symptom
canavan disease
carcinoma of the gallbladder
coats disease
congenital diaphragmatic hernia
dedifferentiated liposarcoma
epidermolysis bullosa simplex
erythropoietic protoporphyria
esophageal adenocarcinoma
hodgkin lymphoma, classical
severe combined immunodeficiency
werner syndrome
x-linked adrenoleukodystrophy
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