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Expression of N-acetylglucosaminyltransferase V in the subserosal layer correlates with postsurgical survival of pathological tumor stage 2 carcinoma of the gallbladder.
[carcinoma of the gallbladder]
N-Acetylglucosaminyltransferase
V
(
GnT-V
)
,
an
enzyme
that
catalyzes
the
β
1
-
6
branching
of
N-
acetylglucosamine
on
asparagine-linked
oligosaccharides
of
cellular
proteins
,
enhances
the
malignant
behaviors
of
carcinoma
cells
in
experimental
models
.
The
aim
of
this
study
was
to
determine
clinical
significance
of
GnT-V
expression
in
human
pT
2
gallbladder
carcinoma
with
simple
in
vitro
experiments
.
Ninety
patients
with
pT
2
gallbladder
carcinoma
were
included
for
this
study
.
The
in
vitro
and
in
vivo
biological
effects
of
GnT-V
were
investigated
using
gallbladder
carcinoma
cells
with
variable
GnT-V
expression
levels
induced
by
a
small
interfering
RNA
.
Of
the
90
cases
,
57
showed
positive
staining
and
the
remaining
33
demonstrated
negative
staining
,
the
subcellular
localization
in
the
57
cases
was
classified
into
the
granular-
type
in
31
cases
and
the
diffuse
-
type
in
26
cases
.
In
76
cases
with
curative
resection
,
postsurgical
survival
was
significantly
poorer
in
those
showing
positive
staining
than
in
those
showing
negative
staining
(
P
=
0
.
028
)
.
In
all
of
the
76
cases
,
postsurgical
recurrence
was
significantly
more
frequent
in
those
showing
diffuse
-
type
localization
than
in
those
showing
negative
staining
.
Experimental
analyses
demonstrated
that
the
down-regulation
of
GnT-V
expression
in
gallbladder
carcinoma
cells
induced
suppression
of
cell
growth
in
vitro
.
The
expression
levels
of
GnT-V
in
the
cells
were
highly
correlated
with
the
rapid
in
vivo
growth
coupled
with
the
enhanced
angiogenesis
,
and
the
tendency
to
form
liver
metastasis
.
GnT-V
expression
in
the
subserosal
layer
of
pT
2
gallbladder
carcinoma
is
correlated
with
the
aggressiveness
of
the
disease
.
